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KMID : 0379520050210040355
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한국독성학회지
2005 Volume.21 No. 4 p.355 ~ p.360
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Inhibition of Cell Proliferation and Induction of Apoptosis by Diallyl Disulfide in Human Colon Cancer Cell lines
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Kim Tae-Myung
Ryu Jae-Myun
Kwon Hyun-Jung
Woo Koan-Sik
Jeong Heon-Sang
Hong Jin-Tae
Kim Dae-Joong
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Abstract
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Epidemiological and laboratory studies provide insight into the anti-carcinogenic potential of garlic and its constituent compounds. Garlic is appealing as an anti-carcinogenic agent due to its ability to induce apoptosis in vitro. Diallyl disulfide (DADS) is one of the major components of garlic that used to determine inhibition of cell proliferation and induced apoptosis in human colon cell lines. In this study, human colorectal cancer cell lines (LOVO, HCT-116, SW-480) were exposed to DADS. The inhibitory effects of DADS dose level more than 50;{mu}M in the cell viability of all cell lines. Cell growth activity inhibits of human colon cancer cell lines. The inhibitory effects of DADS dose level more than $25~50;{mu}M in the cell growth using MTT assay. We found that DADS may have the apoptosis action (chromatin condensation, DNA fragmentation) using DAPI staining and increased the expression of caspase-3 at the dose level more than 100;{mu}M, decreased the expression level of beta-catenin at dose dependent in the western blotting. We suggest that DADS may have a potential candidate as cancer chemopreventive agents.
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KEYWORD
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Cancer chemopreventive agent, Garlic, Diallyl disulfide (DADS), Human colorectal cancer cell lines (LOVO, HCT-116, SW480), Apoptosis
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